Altered-States.net

Pemf and Stem Cells

We are, only as healthy as our cells.

To Have healthy cells in our present pollurted climate, one has to take an active process. Regular tun-up of our cells is not only feasible, but also necessary to slow aging and reduce the risk of cell dysfunction.

"Stem cells are a type of cell that has the potential to develop into different cell types in the body. Because of this, these types of cells can serve as a repair system and theoretically divide without limit to replenish other cells for as long as you are alive. When a stem cell divides, each “daughter” cell has the potential to either remain a stem cell or become another type of cell with a more specialized function, such as a heart muscle cell, a red blood cell, or a brain cell. In theory, we might want to simply boost stem cells while we are young and then use them to keep our heart, hips, brain, and other parts our body healthy as we age"

Stem Cell Enhancement

Stem cells have the remarkable potential to develop into many different cell types in the body. Serving as a sort of repair system for the body, they can theoretically divide without limit to replenish other cells as long as the person or animal is still alive. When a stem cell divides, each new cell has the potential to either remain a stem cell or become another type of cell with a more specialized function, such as a muscle cell, a red blood cell, or a brain cell.

Stem cells are cells found in all multi-cellular organisms. They retain the ability to renew themselves through mitotic cell division and can differentiate into a diverse range of specialized cell types. Research in the stem cell field grew out of findings by Canadian scientists Ernest A. McCulloch and James E. Till in the 1960s

Stem cell research has been hailed for the potential to revolutionize the future of medicine with the ability to regenerate damaged and diseased organs. This article presents an overview of what stem cells are, what roles they play in normal processes such as development and cancer, and how stem cells could have the potential to treat incurable diseases.

The Effects of a Pulsed Electromagnetic Field on the Proliferation and Osteogenic Differentiation of Human Adipose-Derived Stem Cells

Conclusions

These results suggest that PEMF can significantly promote the proliferation and osteogenic differentiation of adipose-derived stem cells so as to achieve the purpose of treating fractures and large bone defects. hASCs also have the same osteogenic differentiation potential as BMSCs. These findings can provide insight into the development of PEMF as an effective technique for regenerative medicine. Source

Stem cell research has been hailed for the potential to revolutionize the future of medicine with the ability to regenerate damaged and diseased organs. On the other hand, stem cell research has been highly controversial due to the ethical issues concerned with the culture and use of stem cells derived from human embryos. This article presents an overview of what stem cells are, what roles they play in normal processes such as development and cancer, and how stem cells could have the potential to treat incurable diseases.

In addition to offering unprecedented hope in treating many debilitating diseases, stem cells have advanced our understanding of basic biological processes.

Three processes in which stem cells play a central role in an organism, development, repair of damaged tissue, and cancer resulting from stem cell division going awry.

Research and clinical applications of cultured stem cells: this includes the types of stem cells used, their characteristics, and the uses of stem cells in studying biological processes, drug development and stem cell therapy; heart disease, diabetes and Parkinson's disease are used as examples.

During development, stem cells divide and produce more specialized cells. Stem cells are also present in the adult in far lesser numbers. The role of adult stem cells (also called somatic stem cells) is believed to be replacement of damaged and injured tissue. Observed in continually-replenished cells such as blood cells and skin cells, stem cells have recently been found in other tissue, such as neural tissue.

Pulsed electromagnetic fields potentiate the paracrine function of mesenchymal stem cells for cartilage regeneration

Conclusions
We provide evidence that brief exposure to low amplitude PEMFs enhanced the ability of MSCs to produce and secrete paracrine factors capable of promoting cartilage regeneration as well as protecting against adverse inflammatory conditions. Furthermore, this report highlights the importance of optimizing PEMF exposure parameters for MSCs subjected to different culturing conditions. Collectively, our results indicate that PEMF stimulation could augment the production and release of the MSC paracrine repertoire for the ultimate enhancement of cartilage regeneration. Source

Organ regeneration has long been believed to be through organ-specific and tissue-specific stem cells. Hematopoietic stem cells were believed to replenish blood cells, stem cells of the gut to replace cells of the gut and so on. Recently, using cell lineage tracking, stem cells from one organ have been discovered that divide to form cells of another organ. Hematopoietic stem cells can give rise to liver, brain and kidney cells. This plasticity of adult stem cells has been observed not only under experimental conditions, but also in people who have received bone marrow transplants.4

For wound healing in the skin, epidermal stem cells and bone-marrow progenitor cells both contribute.6 Thus it is likely that organ-specific progenitors and hematopoietic stem cells are involved in repair, even for other organ repair.

No other cell in the body has the natural ability to generate new cell types

Pulsed Electromagnetic Field Therapy (PEMF) is a therapy that influences cell behavior by inducing electrical changes to occur that stimulates cellular regeneration resulting in accelerated healing of damaged tissue, decreased inflammation, pain relief and greater range of motion with NO adverse reactions.

" PEMF Therapy detoxifies the body at a cellular level. Healthy cell metabolism is required to produce energy and maintain cellular health. Pulser electro magnetic fields induces electrical changes within the cell that boosts cellular metabolism, regenerates blood cells, improves circulation, increases the pH, enhances stem cell production, increases cell hydration and increases the cellular level of oxygen absorption by up to 200%. If the cells are healthier then the immune system becomes stronger, the nervous system relaxes, bones, joints and vital organs become stronger…ultimately, giving the body the environment to heal and function as God intended."

NASA has invested billions of dollars into space program research (including stem cell research) during the last 20 years, involving multiple tests in space shuttles.

NASA found that specific magnetic fields increased stem cell growth and NASA found the exact magnetic field that optimizes stem cell growth up to 4 fold and increased about 120 tissue growth and regeneration genes.

NASA Also discovered about a 400% increase in neural stem cells and
turned on about 160 genes that control growth and regeneration

10 hertz stimulation has been studied by NASA on stem cells (Goodwin). With their particular 10 Hz signal, NASA discovered about a 400% increase in neural stem cells and turned on about 160 genes that control growth and regeneration

Teath And Gums

In addition, research in Germany (Wever ) found that 10 Hz stabilized circadian rhythms. Use of this frequency can restore jet lag and other sleep disturbances.

All cells need energy to function. Adenosine triphosphate, created using food or sunlight, is the molecule that cells use to store that energy for future use.Adenosine triphosphate, also known as ATP, is the energy molecule of all living cells. When plants get energy from sunlight and animals get energy from food, this energy must be converted to ATP before it can be used by the cells to carry out all necessary functions.

A 10 Hz pulsed (square-wave) field increases cell's organic production of ATP (the fuel that fires all cellular processes). This is the premise behind the theoretical proposition we call instantATP (organisms under the influence of pulsed electromagnetic fields 10 Hz)

When cells are moderately low in ATP, the major problem may be fatigue or exercise intolerance. [Think of a car engine. If the gas in the tank is good fuel, the engine runs well. If the gas has water or sand in it, the car runs poorly.] Rhabdomyolysis (muscle cell death) occurs when the cells don’t have enough ATP (that is, the car runs out of gas). Constant or on-going weakness may develop as a result of repeated low-grade rhabdomyolysis.

NASA Uses Frequencies to Enhance Neural Tissue

Regeneration of nerve cells using 10 hz square EM fields

In 2003 NASA-Goodwin found 10 Hz square wave stimulation caused neural tissue regeneration @ 4x baseline, w/better 3-D orientation; cell DNA signature reverted from maturation to developmental (more than 175 maturation genes and 150 developmental genes pgs. 15-18)..

Cells that are mature are literally "tricked" into believing they are younger than they are. Mitochondrial DNA (mtDNA) may have been repaired through some unidentified mechanism or to adequate detoxification of mitochondrion itself or cell protoplasm within which the mitochondria reside

In NASA study above, mitochondrial robustness due to 10 Hz stimulation provided the energy to support regeneration at 4 times baseline. There is related information in that author's patents at US 6485963 and 6673597, both of which are titled "Growth stimulation of biological cells and tissue by electromagnetic fields and uses thereof".

NASA's CONCLUSION:
" We have clearly demonstrated the bioelectric/biochemical potentiation of nerve stimulation and restoration in humans as a documented reality".
Final Recommendation: "One may use square wave EM fields for":

a. repairing traumatized tissues
b. moderating some neurodegenerative diseases
c. developing tissues for transplantation

*the first study to clarify technologies and efficacy parameters for tissue growth and restoration

Exposure to 10 Hz has an immediate and direct effect on the mitochondria as demonstrated in the studies above. Pure organic ATP according to biological paradigm on planet Earth in both plants and animals.

A 10 Hz pulsed (square-wave) field increases cell's organic production of ATP (the fuel that fires all cellular processes). This is the premise behind the theoretical proposition we call instantATP (organisms under the influence of pulsed electromagnetic fields 10 Hz).

Introducingt magnetic field to the human body is likewise expected to stimulate production of stem cells. This stimulation would allow the body to perform rapid ‘self repair’ not only in local tissues but also in the whole body. Read The NASA study here

Tools for PEMF stimulation using the NASA research which found: that specific magnetic
fields increased stem cell growth

The electrical square waves are single electrical pulses alternating positive -> negative -> positive -> … with a 200 ms delay between each pulse to yield 5-10 Hz pulses per second.

Simulated 5-10 Hz electrical square waves tuned to produce the necessary magnetic field waves between the 2 coils.

This protocol can be achieved with the rife opton 3h - 3ah system here using the Rife amplifier and the twin coil system

Few quarrel with predictions of the awesome potential that stem cell research holds. One day, scientists say, stem cells may be used to replace or repair damaged cells, and have the potential to drastically change the treatment of conditions like cancer, Alzheimer's and Parkinson's disease and even paralysis

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For ethical issues and stem cell research refer to http://stemcells.nih.gov/info/ethics.asp; Ethical Issues Associated with Pluripotent Stem Cells. Human Embryonic Stem Cells (2003) ed. by Chiu A.Y., Rao, M.S, 3-25.
Sell, S. (2004) Stem cells. Stem Cell Handbook ed. by Sell, S. 1-18.
Forbes, S.J., Vig, P., Poulsom, R., Wright, N.A., Alison, M.R. (2002) Adult Stem Cell Plasticity: New Pathways of Tissue Regeneration become Visible. Clin. Sci. 103, 355-369.
Asahara T., Isner, J.M. (2004) Endothelial Progenitor Cells. Stem Cell Handbook ed. by Sell, S. 221-227.

Lindblad, W.J. (2004) Stem cells in Dermal Wound Healing. Stem Cell Handbook ed. by Sell, S. 101-105.
McCulloch, E.A. (2004) Normal and Leukemic Hematopietic Stem cells and Lineages. Stem Cell Handbook ed. by Sell, S. 119-131.
Tsai, R.Y.L. (2004) A Molecular View of Stem Cell and Cancer Cell Self-renewal. Intl. J. Biochem. Cell Biol. 36, 684-694.
Cai, J., Weiss M.L., Rao, M.S. (2004) In Search of "stemness". Exp. Hematol. 32, 585-598.
Roach, M.L., McNeish, J.D. (2002) Methods for the Isolation and Maintenance of Murine Embryonic Stem Cells. Embryonic Stem Cells Methods and Protocols ed. by Turksen K. 1-16.
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Evans, M.J., Kaufman, M.H. (1981) Establishment in Culture of Pluripotenial Cells from Mouse Embryos. Nature 292, 154-156; Axelrod, H.R. (1984) Embryonic Stem Cell Lines Derived from Blastocysts by a Simplified Technique. Dev. Biol. 101, 225-228; Wobus, A.M., Holzhausen H., Jakel, P., Schneich, J. (1984) Characterization of a Pluripotent Stem Cell Line Derived from a Mouse Embryo. Exp. Cell Res. 152, 212-219; Doetschman, T.C. Eistattaer, H., Katz, M., Schmidt, W., and Kemler, R. (1985) The in vitro development of Blastocyst Derived Embryonic Stem Cell Lines: formation of Yolk Sac, Blood Islands and Myocardium. J. Embryol. Exp. Morphol. 87, 27-45.
Thompson, J.A., Kalishman, J., Golos, T.G., Durning, M., Harris, C.P., Becker, R.A., Hearn, J.P. (1995) Isolation of a Primate Embryonic Stem Cell Line. Proc. Natl. Acad. Sci. USA 86, 7844-7848; Thomson, J.A, Itskovitz-Eldor, J., Shapiro, S.S., Waknitz, M.A., Swiergiel, J.J., Marshal, V.S., Jones, J.M. (1998) Embryonic Stem Cell Lines Derived from Human Blastocysts. Science 282, 1145-1147.
Amit, M., Segev, H., Manor, D., Itskovitz-Eldor, J. (2003) Subcloning and Alternative Methods for the Derivation and Culture of Human Embryonic Stem Cells. Human Embyronic Stem Cells ed. by Chiu, M., Rao, M.S. 127-141.
Carpenter, M.K., Xu, C., Daigh, C.A., Antosiewicz, J.E., Thomson, J.A. (2003) Protocols for the Isolation and Maintenance of Human Embryonic Stem Cells. Human Embyronic Stem Cells ed. by Chiu, M., Rao, M.S.
Drukker M., Benvenisty, N. (2003) Genetic Manipulation of Human Embryonic Stem Cells. Human Embryonic Stem Cells ed. by Chiu, A.Y., Rao, M.S. 265-284.

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Shamblott, M.J., Axelman, J., Wang, S., Bugg, E.M., Littlefield, J.W., Donovan, P.J., Blumenthal, P.D., Huggins, G. R., Gearhart J.D., (1998) Derivation of Pluripotent Stem Cells from Cultured Human Primordial Germ CellS. Proc. Natl. Acad. Sci.USA 95, 13726-13731.
Doyonnas, R., Blau, H.M. (2004) What is the Future of Stem Cell Research? Stem Cell Handbook ed. by Sell, S. 491-499.
Draper, J.S. Moore, H., Andrews, P.W. (2003) Embryonal Carcinoma Cells. Human Embryonic Stem Cells ed. Chiu, A. Y., Rao, M.S. 63-87.
Adult Stem Cells ed. Turksen, K. (2004) Nosrat, I.V., Smith, C. A., Mullally, P., Olson, L., Nosrat C.A. (2004) Dental Pulp Cells Provide Neurotrophic Support for Dopaminergic Neurons and Differentiate into Neurons in vitro; implications for Tissue Engineering and Repair in the Nervous System. Eur. J. of Neurosci. 19, 2388-2398.
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Shen, C-N., Horb, M.E., Slack, J.M.W., Tosh,D. (2003) Transdifferentiation of Pancreas to Liver. Mech. Dev.120, 107-116.
Priller, J. (2004) From Marrow to Brain. Adult Stem Cells ed. by Turksen, K. 215-233.
de Wynter, E.A. (2003) What is the future of Cord blood stem cells? Cytotech. 41, 133-138.
Gossler, A., Doetschman, T.C., Eistattaer, H., Katz, M., Schmidt, W., Kemler, R. (1986) Transgenesis by means of Blastocyst Derived Embryonic Stem Cell Lines. Proc. Natl. Acad. Sci. USA 83, 9065-9069.
Thomas, K.R., Capecchi, M.R. (1987) Site-directed Mutagenesis by Gene Targeting in Mouse Embryo-derived Stem Cells. Cell 51, 503-512.; Koller, B.H., Hageman, L.J., Doetschman, T.C., Hagaman, J.R., Huang, S., Williams, P.J., et. al. (1989) Proc. Natl. Acad. Sci. USA 86, 8924-8931.
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For review: Floss,T., Wurst, W. (2002) Functional Genomics by Gene-trapping in ES cells. Embryonic Stem Cells Methods and Protocols ed. by Turksen, K. 347-379.
McNeish, J. (2004) Embryonic Stem Cells in Drug Discovery Nat. Rev. Drug Discov. 3, 70-80.
Davila, J.C., Cezar, G.G., Thiede, M., Strom, S., Miki, T., Trosko J. (2004) Use and Application of Stem Cells in Toxicology. Toxicol. Sci. 79, 214-223.
Till, J.E., McCulloch, E.A. (1961) A Direct Measurement of the Radiation Sensitivity of Normal Mouse Bone Marrow Cells. Radiat. Res. 14, 2213-222.
Thomas, E.D. (1999) Bone Marrow Transplantation: a Review. Semin. Hematol. 36, 95-103.
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Barker, R.A., Jain, M., Armstrong, R.J.E., Caldwell, M.A. (2003) Stem Cells and Neurological Disease. J. Neurol. Neurosurg. Psychiat. 74, 553-557.
Jackson, K.A., Goodell, M.A. (2004) Generation and Stem Cell Repair of Cardiac Tissue. Stem Cell Handbook, edited by Sell, S. 259-266.
Kehat, I., Khimovich, L., Caspi, O., Gepstein, A., Shofti, R., Arbel, G., Huber, I., Satin, J., Itskovitz-Eldor, J., Gepstein, L. (2004) Electromechanical Integration of Cardiomyocytes Derived from Human Embryonic Stem Cells . Nature Biotechnol. 22, 1282-1289.
Fraser, J.K., Schreiber, R.E., Zuk, P.A., Hedrick, M.H. (2004) Adult Stem Cell Therapy for the Heart. Intl. J. Biochem. Cell Biol. 36, 658-666.
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Cohen, S., Leor, J. (2004) Rebuilding Broken Hearts. Scientific American Nov. 2004, 45-51.
Street, C.N., Sipione, S., Helms, L., Binette, T., Rajotte, R.V., Bleackley, R.C., Korbutt, G.S. (2004) Stem Cell-based Approaches to Solving the Problem of Tissue Supply for Islet Transplantation in Type I Diabetes. Intl. J. Biochem. Cell Biol. 36, 667-683.
Bouwens, L. (2004) Islet Cells. Stem Cell Handbook ed. by Sell, S. 429-438.
Seaberg, R.M., Smukler, S.R., Kieeffer, T.J., Enikolopov, G., Asghar, Z., Wheeler M.B., Korbutt, G., van der Kooy, D. (2004) Clonal Identification of Multipotent Precursors from Adult Mouse Pancreas that Generate Neural and Pancreatic Lineages. Nat. Biotechnol. 22, 1115-1124.; SeNakajima-Nagata, N., Sakurai, T., Mitaka, T., Katakai, T., Yamaot, E., Miyazaki, J., Tabata, Y., Sugai, M., Shimzu, A.. (2004) In vitro Induction of Adult Hepatic Progenitor Cells into Insulin-producing Cells. Biochem. Biophys. Res. Commun. 318, 625-630.
Baier, P.C., Schindehutte HJ., Thinane, K., Flugge G., Fuchs, E., Mansouri, A., Paulus, W., Gruss, P.,Trenwalder, C.(2004) Behavioral Changes in Unilaterally 6-Hydroxy-Dopamine Lesioned Rats after Transplantation of Differentiated Mouse Embryonic Stem Cells without Morphological Integration. Stem Cells 22, 396-404.
Lindvall O., Bjorklund, A. (2004) Cell Therapy in Parkinson's Disease. NeuroRx. 1, 382-393.
Zheng, X., Cai, J., Chen, J., Luo, Y., Zhi-Bing Y., Fotter, E., Wang, Y., Harvey, B., Miura, T., Backman, C., Chen, G-J., Rao, M.S., Freed. W.J. (2004) Dopaminergic Differentiation of Human Embryonic Stem Cells. Stem Cells 22, 925-940; Wilmut, I., Paterson, L.A. (2004) Stem cells and Cloning. Stem Cell Handbook ed. by Sell, S. 75-80.
Barker, R.A., Widner, H. (2004). Immune Problems in the Central Nervous System Cell Therapy. NeuroRx. 1, 472-481.