1. Ozone Selectively Inhibits Growth of Human Cancer Cells

From Science magazine Vol. 209, 22 Aug 1980, pp. 931-933


The growth of human cancer cells from lung, breast, and uterine tumors was selectively inhibited in a dose-dependent manner by ozone at 0.3 to 0.8 part per million of ozone in ambient air during 8 days of culture. Human lung diploid fibroblasts served as noncancerous control cells. The presence of ozone at 0.3 to 0.5 part per million inhibited cancer cell growth 40 and 60 percent, respectively. The non-cancerous lung cells were unaffected at these levels. Exposure to ozone at 0.8 part per million inhibited cancer cell growth more than 90 percent and control cell growth less than 50 percent. Evidently, the mechanisms for defense against ozone damage are impaired in human cancer cells.

PMID: 7403859 [PubMed - indexed for MEDLINE]

2. Ozone Therapy for Tumor Oxygenation: a Pilot Study**

Authors: Bernardino Clavo1; Juan L. Pérez2; Laura López1; Gerardo Suárez1; Marta Lloret1; Victor Rodríguez3; David Macías2; Maite Santana1; María A. Hernández1; Roberto Martín-Oliva2; Francisco Robaina4

Source: Evidence-based Complementary and Alternative Medicine, Volume 1, Number 1, 01 June 2004, pp. 93-98(6) Publisher: Oxford University Press


Tumor hypoxia is an adverse factor for chemotherapy and radiotherapy. Ozone therapy is a non-conventional form of medicine that has been used successfully in the treatment of ischemic disorders. This prospective study was designed to assess the effect of ozone therapy on tumor oxygenation. Eighteen subjects were recruited for the study. Systemic ozone therapy was administered by autohemotransfusion on three alternate days over one week. Tumor oxygenation levels were measured using polarographic needle probes before and after the first and the third ozone therapy session. Overall, no statistically significant change was observed in the tumor oxygenation in the 18 patients. However, a significant decrease was observed in hypoxic values 10 and 5 mmHg of pO<inf>2</inf>. When individually assessed, a significant and inverse non-linear correlation was observed between increase in oxygenation and the initial tumor pO<inf>2</inf> values at each measuring time-point, thus indicating that the more poorly-oxygenated tumors benefited most (rho = -0.725; P = 0.001). Additionally, the effect of ozone therapy was found to be lower in patients with higher hemoglobin concentrations (rho = -0.531; P < 0.034). Despite being administered over a very short period, ozone therapy improved oxygenation in the most hypoxic tumors. Ozone therapy as adjuvant in chemo-radiotherapy warrants further research.

Document Type: Research article

DOI: 10.1093/ecam/neh009

Affiliations: 1: Radiation Oncology and Research Unit, Las Palmas (Canary Islands), Spain 2: Medical Physics, Las Palmas (Canary Islands), Spain 3: La Paterna Medical Center, Las Palmas (Canary Islands), Spain 4: Chronic Pain Unit, Dr Negrín Hospital, Las Palmas (Canary Islands), Spain

3. Ozone Plus Cobalt Therapy In Patients Suffering From Prostatic Cancer

Reported at the 2nd International Symposium on Ozone Applications: March 1997 by L. Borrego, L. L. Borrero, E. C. Díaz1, S. Menéndez2, L. R. Borrego3, R. A. Borrego3. This study involved these institutes in Cuba: Provincial Center of Retinitis Pigmentosa, Holgu'n, V.I. Provincial Center of Retinitis Pigmentosa, Holguín, Ozone Research Center.

Prostatic adenocarcinoma is the most frequent tumor in men older than 50 years old, representing the 16.7 % of all the death cases of cancer in male sex. With an early diagnose and an appropriate treatment the survival increase in 5 years, in 91 % of cases. Cancer metabolism is fundamentally anaerobic, for that reason, increasing oxygenation in damaged tissues, may diminished the side effects produced by radiation treatments, as well as increase radio-sensibility. The aim of this study is to evaluate the efficacy of ozone therapy in conjunction with cobalt-60 therapy, in the treatment of patients suffering from prostatic cancer, taking into account the influence that ozone exerts in tissue oxygenation. 70 patients with malignant neoplasia in prostate, stage A and B of the disease (intracapsular) were treated by an oncourology muldisciplinary group of the "Lenin" Hospital (Holguin Province). 35 patients were treated with rectal ozone plus cobalt therapy (ozone group) and 35 with cobalt therapy only (control group). The appearance of side effects (dermatitis radiation, cystitis, proctitis) and the behavior of some humoral and clinical parameters were evaluated up to 6 months after finishing the treatment. 84 % and 52 % of side effects were presented in control group and ozone group, respectively. Prostatic specific antigen (PSA) decrease, less than 10 ng/mL, in 92 % of patients treated with ozone and in 52 % in control group,
one month after finishing the treatment. 88 % and 80 % of clinical and humoral control of the disease were obtained in ozone group and control group, respectively, 6 months later. It is concluded that, combining cobalt therapy with ozone, a decrease in side effects and an increase in radio-sensibility were achieved.

4. Adjuvant Ozonetherapy in Advanced Head and Neck Tumors: A Comparative Study **

Clavo B, Ruiz A, Lloret M, Lopez L, Suarez G, Macias D, Rodriguez V, Hernandez MA, Martin-Oliva R, Quintero S, Cuyas JM, Robaina F.

Advanced head and neck (H&N) tumors have a poor prognosis, and this is worsened by the occurrence of hypoxia and ischemia in the tumors. Ozonetherapy has proved useful in the treatment of ischemic syndromes, and several studies have described a potential increase of oxygenation in tissues and tumors. The aim of this prospective study was to evaluate the clinical effect of ozonetherapy in patients with advanced H&N cancer in the course of their scheduled radiotherapy. Over a period of 3 years, 19 patients with advanced H&N tumors who were undergoing treatment in our department with non-standard fractionated radiotherapy plus oral tegafur. A group of 12 patients was additionally treated with intravenous chemotherapy before and/or during radiotherapy. In the other group of seven patients, systemic ozonetherapy was administered twice weekly during radiotherapy. The ozonetherapy group was older (64 versus 54 years old, P = 0.006), with a higher percentage of lymph node involvement (71% versus 8%, P = 0.019) and with a trend to more unfavorable tumor stage (57% versus 8% IVb + IVc stages, P =
0.073). However, there was no significant difference in overall survival between the chemotherapy (median 6 months) and ozonetherapy (8 months) groups. Although these results have to be viewed with caution because of the limited number of patients, they suggest that ozonetherapy could have had some positive effect during the treatment of our patients with advanced H&N tumors. The adjuvant administration of ozonetherapy during the chemo-radiotherapy for these tumors merits further research.

PMID: 15841266 [PubMed - as supplied by publisher]

5. Effect of a parenteral ozone-oxygen mixture on the concentration of immunoglobulins (IgA, IgG, IgM), of vitamin A and lysozyme activity in patients with cervical cancer**

Geburtshilfe Frauenheilkd. 1987 Dec;47(12):343-5 written in German

Enzelsberger H , Metka M , Salzer H .

Univ.-Frauenklinik Wien.

In the literature you can find several therapeutic possibilities in the treatment of carcinoma by additive ozone therapy. We investigated the consequences of ozone therapy for the immunological status, lysozyme and vitamin A. 21 women with progressive cervical cancer (Stage III, IV) got besides the conventional irradiation therapy also an additive ozone therapy. After irradiation and ozone therapy a small decrease in IgG, IgA and IgM can be seen. A statistical significance could not be evaluated. There was no difference to the control group in lysozyme and vitamin A.

PMID: 3436505 [PubMed - indexed for MEDLINE]

6. Intravenous injection therapy for genital cancer with an ozone-oxygen mixture in gynecological radiotherapy**

Ozon-Sauerstoff-Injektionsbehandlung in der gynäkologischen Strahlentherapie
Hernuss, P.; E. Müller-Tyl & J. Dimopoulos.
Strahlentherapeutische Klinik der Universität Wien, A-1097 Wien, Alserstraße 4, Austria.
Strahlentherapie 148(3): 242-245, 1974.

Written in German


40 female patients with primary and 5 patients with recurrence of genital cancer after treatment have received radiotherapy and a daily parenteral ozone mixture medication. It appears that under influence of the supplementary ozone-oxygen mixture treatment, the regression of female genital tumors in both groups studied was faster. The side-effects of the radiotherapy were reduced and
the general condition of the patients improved. The best results were observed in patients with recurrence of poorly oxygen-supplied genital tumors.

7. Ozone and gynecologic radiotherapy**

Original study written in German and titled: Ozon und gynakologische Strahlentherapie

Hernuss P; Muller-Tyl E; Wicke L

Strahlentherapie (1975 Nov), 150(5), 493-9. ISSN:0039-2073

PubMed ID 1216254


A short survey is given of the historical development and the physical basis of ozone therapy. Ozone is used in medicine as well as in other spheres. Papers reporting good results of ozone treatment in carcinoma seemed of particular interest. The efficacy of ozone as an adjuvant to the irradiation of carcinosarcomas of rats was confirmed by us. On account of this fact ozone was introduced by us as an adjuvant to the irradiation of women with gynaecological cancer and appeared to give good results. The mechanism of action of ozone is not yet fully clarified and several theories are discussed. Investigations are currently being undertaken in respect to the behaviour of several substances in the organism during ozone therapy.

8. Cancer outcomes at the Hufeland: a best-case series review **

(the Hufeland is a complementary/alternative medicine institute)

Integr Cancer Ther. 2005 Jun;4(2):156-67.

Jacobson JS, Grann VR, Gnatt MA, Hibshoosh H, Austin JH, Millar WS, Neugut AI.

Department of Epidemiology, Mailman School of Public Health, New York, NY 10032, USA.

A best-case series review is an efficient tool with which to screen complex complementary and alternative treatments for cancer as candidates for further study. STUDY DESIGN: The National Cancer Institute and other agencies have adopted the best-case series method to evaluate cancer treatments involving complementary and alternative medicine (CAM) for further study. The authors conducted a best-case series review of the Hufeland Klinik. Established in 1985 in Bad Mergentheim, Germany, this facility treats more than 500 cancer patients per year. Hufeland treatment includes dietary modification, injections, ozone therapy, active fever therapy, psychotherapy, and sometimes hormone therapy and/or low-dose chemotherapy. The goal of the treatment is to prolong survival and to maintain good quality of life. METHODS: The clinic provided summaries of 27 cases in which patients with longer than expected survival had agreed to make their medical records available for review. The review involved pathologic confirmation of disease and radiologic confirmation of complete response (CR) or partial response (PR) not attributable to conventional treatment. RESULTS: Based on the summaries and an exhaustive 2-year search for medical records, slides, and imaging data, 12 of 27 cases were selected for full review, and 5 (3 CRs and 2 PRs) were judged best cases. CONCLUSION: Most patients with common cancers receive conventional treatment before coming to Hufeland, and many are treated with chemotherapy and/or hormonal therapy while there. Hence, only a few could be considered for review. With 5 of 12 patients showing a treatment response, the authors conclude that the Hufeland treatment merits further study. They also recommend the development of criteria with which to evaluate best-case series reviews of complex CAM treatments for patients with advanced cancer.

PMID: 15911928 [PubMed - indexed for MEDLINE]

9. Ozonetherapy in a Complex Treatment of Breast Cancer

Kontorschikova, Claudia N.1; Anna V. Alaysova & Igor G. Terentiev.

Chair of clinical and and Laboratory Diagnostics, Department of Oncology, Nizhni Novgorod State Medical Academy, 10/1 Minin sq., N. Novgorod, 603005, Russia.

In: Proceedings of the 15th Ozone World Congress, 11th - 15th September 2001, Medical Therapy Conference (IOA 2001, Ed.), Speedprint Macmedia Ltd, Ealing, London, UK, 2001.


There have been followed up 52 women with breast cancer, confirmed histologically, age ranging from 40 to 60 years. 32 patients along with cytostatic therapy have undergone a course of ozone therapy of intravenous infusions or rectal insufflations and ozonated water per os. 20 women were on conventional polychemotherapy. The groups were compatible according the age, stage of the disease and accompanying pathology. Involvement of ozone therapy in a complex treatment of patients with breast cancer helped to diminish the incidence and degree of cytostatics toxical side effects, improve their life quality and immunological parameters and significally increase the activity of antioxidant defence system


10. Three case reports on the holistic approach to patients with neoplasm including the use of medical ozone**

Thomas Marshall-Manifold, Wimbledon Clinic of Natural Medicine, London, UK.

In this report we present the successful treatment of three patients with malignant carcinoma with a combined alternative therapy including Ozone, diet, Homoeopathy, herbs and immunotherapy. Although treatment with Ozone is not a very aggressive procedure as compared to a large number of conventional measures, usually other traditional alternative therapies such as Homoeopathy and herbs are first given to our patients. Then, the patient's response is evaluated and Ozone is added in many cases to the initial treatment scheme. Although positive tests for acid phosphatase were never found in our male patient, P.S.A. levels were highly suggestive of prostate adenocarcinoma which clearly responded to therapy on two occasions. There is a lot of controversy now on whether the benefits of aggressive treatment in prostate cancer outweigh the associated risks and side effects. Conventional aggressive treatment include radiation and surgery. A fair percentage of patients who undergo this kind of treatment suffer from complications such as impotency (30-50%), mild to severe incontinence and even death.13 In the case of a residual ovarian tumour, the recovery of the patient and the return of her lab tests, in particular CA 12.5, to normal values, were clearly associated to the administration of a combined treatment including Ozone as a major component. In our patient with breast cancer, there was a dramatic change in the regional histopathology including the unusual reversion of a metastatic tumour process to a localised disease. This event could only be explained by the intensive therapeutic measures applied soon after the initial pathologic report. It is also remarkable the way the patient responded to a new treatment programme when a new lump had emerged. These events clearly challenge usual protocols adopted for the conventional treatment of cancer. It is not unusual to obtain similar satisfactory results with this alternative treatment programme in other cases of cancer patients in our clinic. However, an appropriate documentation of these cases implies a big economic effort from our patients which unfortunately limits the frequency of clinical reporting. Likewise, it is really difficult to determine the efficiency of Ozone as a single therapeutic measure in cancer patients. It would obviously be unethical to deprive these patients of empirically and even scientifically tested alternative measures such as dietary supplementation in order to establish the real potential of this gas in Oncology.

11. Ozone-oxygen Therapy for Gynecologic Carcinomas**

The effect of parenteral-ozone oxygen mixture administration on free fatty acids and triglycerides in patients with gynecologic carcinomas

Fortschr Med. 1979 Mar 15;97(10):451-4.

Original article written in German

Muller-Tyl E, Salzer H, Reisinger L, Washuttl J, Wurst F.


As some authors suspect that ozone influences the metabolic process of fat, we tried to analyse the influence of an ozone-oxygen gas which was applied parenterally. 40 patients with gynecological cancer received 10 ml ozone-oxygen gas with a content of 450 gamma ozone and venous blood was removed before and 10 minutes after application. The serum was lyophilized and the level of fatty acids and triglycerids was determined by the method of Randerath (1965). a statistically significant decrease of the concentrations was observed after application of ozone. Different theories as to the cause of this action are discussed.

PMID: 369965 [PubMed - indexed for MEDLINE]

12. Ozonotherapy in the Early Postoperative Period in the Surgical Treatment of Lung Cancer**

V.P. Potanin, V.M. Belopukhov, R.M. Taziev, M.Kh. Mustafin, I.D. Khalimov


The ozonotherapy influence on the postoperative treatment in patients after the radical lung resection due to the lung cancer is analyzed. The studies were performed from 1996 to 1999. The patients were divided into two group: the patients of the first group (40 persons) were treated with the standard infusion therapy in the postoperative period, the patients of the second group (160 persons) werw treated with ozoned crystalloids combined with the standard therapy. Due to the ozonotherapy the purulent complications in the second group reduced by a factor of 12,5 and mortality reduced by a factor of 2,6.

13. In Vitro Synergistic Activity of 5-fluorouracil with Low-dose Ozone Against a Chemoresistant Tumor Cell Line and Fresh Human Tumor Cells**

Chemotherapy. 1990;36(2):147-54.

Zanker KS, Kroczek R., Institute of Immunology and Experimental Surgery, University of Witten/Herdecke, FRG.


We followed the concept that the chemical reactivity of ozone depends upon its oxidative properties. Activated oxygen species are causally involved in toxicity of certain chemotherapeutic drugs. We have tested this prediction in human cell cultures either to overcome chemoresistance and/or to increase chemical cytotoxicity. Our results indicate that ozone in combination with 5-fluorouracil (5-FU) makes a 5-FU-resistant cell line susceptible for the combined treatment modality. Furthermore, ozone acts synergistically or at least additive to chemotherapy in different tumor cell suspensions, derived from the breast and the colon.

PMID: 2311442 [PubMed - indexed for MEDLINE]

14. Restoration of Normoxia by Ozone Therapy May Control Neoplastic Growth**

J Altern Complement Med. 2005 Apr;11(2):257-65.

Bocci V, Larini A, Micheli V.

Department of Physiology, University of Siena, Italy. bocci@unisi.it


In contrast to normal tissues, tumors thrive in hypoxic environments. This appears to be because they can metastasize and secrete angiopoietins for enhancing neoangiogenesis and further tumor spread. Thus, during chronic ischemia, normal tissues tend to die, while neoplasms tend to grow. During the past two decades, it has been shown in arteriopathic patients that ozonated autohemotherapy is therapeutically useful because it increases oxygen delivery in hypoxic tissues, leading to normoxia. Although several oxygenation approaches have been tested, none is able to restore normoxia permanently in patients with cancer. We postulate that a prolonged cycle of ozonated autohemotherapy may correct tumor hypoxia, lead to less aggressive tumor behavior, and represent a valid adjuvant during or after chemo- or radiotherapy. Moreover, it may re-equilibrate the chronic oxidative stress and reduce fatigue.


Quantity of Papers
There is a very large number of papers on ozone. The above 14 are only the tip of the iceburg. Searching in PubMed for ozone returns over 8,000 items.

Side Effects and Toxicity
Ozone has been used in Europe for about 50 years. A 1982 German study of 384,775 patients documented only a 0.000005% rate of adverse side effects. This is much , safer than any drug. For example, several thousand people in America die from aspirin each year each year.

The ozone rash happens to 80% of people who apply ozone to the ears. It can be annoying, itchy, and cause lymph fluid containing toxins to appear on the ears. A very few people can also get the rash, or a mild version when “funneling” the skin. The rash is the body eliminating toxins, and will go away when the toxins are gone, leaving fresh healthy skin.

There are some detox symptoms such as low-grade fever, nausea, sore lungs,faintness, flu-like symptoms, or tiredness when starting to use ozone. These symptoms usually subside within a few days, and are replaced with a feeling of well being and energy. These “side effects” are usually just ozone doing it's job, they eventually go away.

If you get a concentrated breath of medical grade ozone to your lungs, it will instantly oxidize all the toxins in your lungs. The natural reaction is coughing. In reality it's a good thing, but the symptoms seem serious. This is with medical grade ozone usually.

Prolonged exposure in a room full of ozone can cause coughing and light headedness. If this happens, a person could just lower the setting on his or her generator and the problem would go away.